New Methods of Functional Imaging in Retinal Diseases
Coordinador: José Paulo Domingues
Phone:+351 239480226
Fax: +351 239480280
Email: jpd@ibili.uc.pt
General Objectives
Our mission is to develop new optical techniques and instrumentation for ophthalmology with emphasis on fundus fluorescence imaging.
Our research activities aim to extend the current range of diagnostic tools available to ophthalmology, by promoting the use of emerging and advanced optical techniques.
Our group is active in the development of instrumentation capable of exploiting the clinical potential of ocular endogenous fluorescence. Target applications are the early detection of ocular pathologies and the use of endogenous fluorescence for functional imaging.
Our main objectives were:
New instrumentation for measuring blood-ocular barriers permeability.
Mapping corneal fluorescence by confocal microscopy Retinal functional imaging using fluorescence.
Development of methods for corneal cell density measurement.
Development of methods for quantitative fluorescence imaging.
New imaging methods for phenotyping photoreceptor degenerations
Main Achievements
A study aimed to quantify differences in corneal cell densities between diabetic patients and healthy controls was concluded and published.
The study found that the cell density in the basal layer of diabetic patients was significantly lower than in healthy controls.
Development and clinical validation of a new solid-state ocular fluorometer for measuring blood-ocular barriers permeability.
Definition and validation of a new method for in vivo corneal cell density measurement by scanning-slit confocal microscopy.
Standard operating procedures for Fundus Autofluorescence (FAF) imaging were defined. FAF imaging was successfully implemented in our Institute.
Adoption of quantitative methods for FAF image analysis.
Dual wave lehgth confocal laser scanning ophthalmoscope for in vivo measurement of macular pigment density.
Application of novel imaging methods that will be valuable to the phenotyping of photoreceptor degenerations.
Establishment of clinical genetic models for phenotyping with imaging approaches.
PI and Ohter Researchers
José Paulo Domingues
Antonio Miguel Morgado
Jasper Van Best
Maria Joao Quadrado
Ph.D. Student
Ana Ferreira