OPTIMIZAÇÃO DO RENDIMENTO DESPORTIVO E RISCO DE SOBRETREINO EM ATLETAS DE ELITE
II. ORGANIZAÇÃO INTERNA
The coordination of this thematic line will ensure all personal and institutional contacts necessary to implement the research projects. The structure is organized into 3 main teams. Regular meetings with the teams will be carried out, for the analysis of the process. For the achievement of the thematic line we constitute 3 main research teams, responsible for:
TEAM 1: The main responsibility is to identify and select pos-graduate students to join the projects. Other functions are related to characterization of the samples, assuring the fulfill of training logs and the URS self report. This team will also be responsible for the implementation of the testing protocols at the laboratory.  IMPLEMENT MAXIMAL AEROBIC ERGOMETER TEST PROTOCOLS: Oxygen uptake (VO2), carbon dioxide output (VCO2), minute ventilation (VE) and respiratory rate (RR) will be measured breath by breath using an open circuit system (Cosmed Quark, Italy). The analyzers were calibrated before each test. The maximal and submaximal values obtained and other ventilator response markers like VO2 kinetics enable to characterize with strong reliability for the performance capacity.  HEART RATE VARIABILITY TEST: The athletes will be submitted to a heart rate variability test at rest (HRV). This test will be conducted on the same day of maximal protocols before the blood collection. Temporal and spectral analysis of HRV will be done with Kubios ® software (Finland). The purpose of this study was to analyze the effect training dynamics over athlete’s nervous system adaption. The procedures are in accordance to the Task Force of the European Society of Cardiology and the North America Society of Pacing and Electrophysiolog (1996).  Monitoring weekly training load: Every training session will be controlled and both volume and intensity will be measure according to the specific sport methodology. Total and mean weekly training load will be quantified. The objective is to observe as accurately as possible the relationship between training load and the other parameters controlled as well as with competition results. In order to enable comparisons across different sports we will privilege the use of perception scales (Cr10Borg) as was proposed by Foster and colleagues (1998)
Team 2: Collection of blood and saliva samples, storage and processing. This team is responsible for the ELISA determination of salivary cortisol, IgA and C-RP and plasma cytokines.  Collect and management of saliva samples: All subjects will be informed about the procedures for this kind of fluid collection. All saliva samples were stored at -80ºC until analysis. The salivary Cortisol, IgA and PCR will be determined by ELISA commercially kits (Sallimetrics LLC, USA). All saliva samples were stored at -80ºC until analysis  Collect and manage peripheral blood samples: Peripheral blood samples of the athletes and controls will be collected by venipuncture of the antecubital vein .Peripheral blood leukocytes, including neutrophils, lymphocytes, and monocytes, were determined using a cell counter (Beckman Coulter T66,USA). Peripheral blood plasma will be aliquot and frozen at -80ºC until analyze for cytokine concentrations (IFN-γ, Il-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-15, IL-1ra, TNF-α) determined using ELISA assays (Invitrogen).
Team 3: This team is responsible for Psychosocial Characterization and determination of the stress factors influencing the cognitive and emotional responses of athletes. Acquired socio-demographic characterization using an original questionnaire designed specifically for this study and standardized instruments. Personality domains and dispositional optimism will be assessed using NEO-PI-R and LOT-R respectively, in order to characterize stable psychological factors influencing emotional and stress responses. Perceived Stress (PSS), Positive and Negative Affects (PANAS), Sleep quality (PSQI) and Life Events occurrence and impact (LES) in all participants.