Publication date: 05-04-2012 10:58
The present invention provides a method and a diagnostic kit for detection and typing of all human papillomavirus (HPV) comprising: nucleic acid amplification of a region of L1 gene using universal HPV primers, treating of the amplified fragment in four independent restriction reactions with different enzymes, namely Pstl. Haelll, Ddel and Rsal, and identification of HPV type by comparison of RFLP patterns with the reference patterns of known genotypes using an HPV genotyping scheme.
|Data de prioridade||18-03-2008|
|Documento de patente|||