NervGen: Nerve Guide Conduit for Peripheral Nerve Regeneration

KEYWORDS: Optical Peripheral Nerve Regeneration; Regenerative Medicine; Polymer; Dextran; Nerve Guide.

The injury of the peripheral nerves are a quite common, especially in the young male population due to accidental traumatic events. The degree of injury may vary, being the most severe cases associated with permanent disability.

We developed a novel polymeric nerve guide tube based on biocompatible and biodegradable polymers for regenerative medicine, namely peripheral nerve regeneration. This guide tube promotes the regeneration of the nerve, while it is reabsorbed (with adjustable rate) by the organism without inducing adverse reactions that could lead to its rejection and subsequent removal with recourse to a second surgery. This tube results from the combination of two biocompatible and biodegradable polymeric materials (dextran e poly(g)-caprolactone) for peripheral nerve regeneration that are approved by the Food and Drug Administration (FDA).


Photopolymerizable characteristics.

Tailored rate of degradation.


Non-acidic degradation material.

Transparency of the tube facilitates suturing.

Adequate biomechanical properties.

Suture resistant structure.

Simple and low cost production technology.

Properties and size of tubes adaptable to the needs.


Peripheral Nerve Regeneration.

Polymeric tube.

Biomedical application (e.g: dental membrane for drug release).

The dextran based nerve guide conduits have a slower degradation ratio in vitro, in comparison with commercially available collagen-based products. Being a biocompatible sugar its degradation products are neutral and do not cause extension of the immune response as proved by the data collected. The final product is completely amorphous, which confers total transparency to the final conduit. In addition, the conduit presents suitable mechanical properties and elasticity facilitating its manipulation and implantation. The conduit is resistant to suturing forming since no cracks or signs of fragmentation are observed.

The technology for tube fabrication is very simple, cost-effective and relatively fast, with a UV crosslinking mean time of 20 minutes. Moreover, the thermal/mechanical properties can be tailored made by using different formulations:

  • Thermal Properties: TGA, DSC and DMTA analysis of the materials;
  • Surface Morphology: Scanning Electron Microscopy of the surface of the prepared nerve guide conduits;
  • Mechanical tests: Tensile tests of the prepared nerve guide conduits (hydrated);
  • Suture tests: Preliminary suture tests in nerve guide conduits;
  • Swelling Capacity: On both membranes and nerve guide conduits in PBS (pH=7.4);
  • In vitro hydrolytic degradation: On both membranes and nerve guide conduits;
  • In vitro: Cytotoxicity evaluation of membranes using human dental pulp stem cells (hDPSCs);
  • In vivo: Biocompatibility evaluation by ISO 10-998-6 SCORE - implantation of membranes in the subcutaneous tissue of rats;
  • In vivo: Implantation of nerve guide conduits in the rat sciatic nerve after neurotmesis injury to injury to evaluate the regeneration of the nerve.


IPR LEGAL STATUS: Protected in several countries

OWNERSHIP: The rights to the technology are held by the University of Coimbra and University of Porto.

COLLABORATION SOUGHT: Licensing for further developments or R&D partnership.



Vanessa Azevedo
Innovation Manager
Laura Alho
IP Manager