II Cell Culture and Tissue Training Course
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Cell
culture has become a essential tool for basic and translational research namely
Medicine, Physiology, Biotechnology, Pharmaceutical or Cellular and
Molecular Biology.
As an excellent alternative for the use of animal models, this technique has
emerged as an essential tool.
This course aims to give theoretical and pratical fundaments of cell and tissue culture techniques.
Theoretical Course
• Principles of cell culture
• Advantages and constraints of using cell cultures
• Problems and troubleshooting in cell cultures
• Proliferation and Viability Assays
• Flow citometry
• Statistics applied to cell culture assays
Pratical Course
• Thawing and Cryopreservation
• Cell Line subculture
• Primary cell cultures
• Contamination detection
• Viability, cytotoxicity and proliferation assays
• Co-cultures
• Database in cell culture assays