III Cell Culture and Tissue Training Course
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A single cell is the building block for life, reason why cell culture has become one of the major tools used in numerous fields of life sciences today. In its simplest form, cell culture is a complex process by which cells are dispersed under controlled conditions, in an artificial environment composed of nutrient solutions, in a suitable surface that allow cells to grow, with ideal conditions of temperature, humidity and gaseous atmosphere. In such a system, a researcher can precisely measure cells response to different alterations and analyze biological properties and processes that are not readily accessible at the level of the intact organism.Cell culture has become an essential tool for basic and translational research namely Medicine, Physiology, Biotechnology, Pharmaceutical or Cellular and Molecular Biology. As an excellent alternative for the use of animal models, this technique has emerged as an essential tool. This course aims to give theoretical and practical fundaments of cell and tissue culture techniques. Therefore, we expect to enjoy this III Cell Culture and Tissue Training Course and take the best of it!
Theoretical course
- Principles of cell culture
- Advantages and constraints of cell culture
- Problems and troubleshooting in cell cultures
- Biosafety levels
- Cell culture models
- Primary cultures
- Organotypic cell cultures
- Flow cytometry
- Microscopy
- Statistics applied to cell culture assays
Practical course
- Thawing and cryopreservation
- Cell lines subculture
- Primary cell cultures
- Contamination detection
- Viability, cytotoxicity and proliferation assays
- Flow cytometry
- Microscopy
- Co-cultures
- Database in cell culture assays
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