Equipment

The 600 MHz NMR Spectrometer is equipped with an 14.1 T Magnex Scientific magnet and a Bruker Avance NEO console with 4 independent radio-frequency observe channels, ZZ-axis pulsed field gradients (PFG), and a helium-cooled cryo platform.

This spectrometer is highly suited for structural studies of macromolecules, in particular biomacromolecules such as proteins and nucleic acids, macromolecule-ligand interactions, and metabolomic studies. Taking advantage of the quadruple resonance (¹H-¹³C-¹⁵N-¹⁹F) cryo-probe design, high sensitivity ¹H-NMR spectra with ¹⁹F decoupling or ¹⁹F-NMR spectra with ¹H decoupling may be collected, in addition to triple resonance experiments including deuterium decoupling for double and triple labeled bio-NMR samples.

Spectrometer Information

• 600 MHz NMR spectrometer with 14.1 T Magnex Scientific magnet and a Bruker Avance NEO console
• helium-cooled cryoprobe platform
• 5 independent broad band RF channels
• ZZ-axis pulsed field gradients (PFG)
• sample temperature control (-20 ºC to 150 ºC)
• TopSpin4 acquisition and processing NMR Software running under Linux CentOS 7

Available Probes

• Bruker BBFO iProbe: 5 mm Double Resonance broad band probe – optimized for both ¹H observation and X-nucleus observation (¹⁹F, ³¹P-¹⁰⁹Ag) with ¹H-decoupling; with automated tuning and matching
• Bruker QCI-F CryoProbe: 5 mm Quadruple Resonance HFCN cryoprobe – optimized for highest sensitivity detection of ¹H, ¹⁹F and ¹³C; with automated tuning and matching; potential applications range from triple resonance including deuterium decoupling for double and triple labeled bio-NMR samples to ¹⁹F{¹H} screening applications and small molecule structure elucidation

The 500 MHz NMR Spectrometer is equipped with 3 radio-frequency observe channels, ZZ-axis pulsed field gradients (PFG), high-resolution magic angle spinning (HR-MAS) accessory, and automatic sample changer with refrigerated sample storage.

This spectrometer is perfectly suited for metabolomic studies based on direct observation of ²H, ¹H, or ¹³C-NMR and routine ¹H and ¹³C-NMR studies of small and mid-size molecules. The HR-MAS accessory and probe allows for solution NMR studies of ex vivo biologic tissues and viscous samples.

Spectrometer Information

• 500 MHz Bruker Avance III HD Spectrometer (11.7 T magnet)
• 3 radio-frequency observe channels
• ability for deuterium NMR observation with fluorine (¹⁹F) lock
• ZZ-axis pulsed field gradients (PFG)
• high-resolution magic angle spinning (HR-MAS) accessory
• automatic sample changer with refrigerated sample storage
• sample temperature control (-20 ºC to 150 ºC)
• TopSpin3 acquisition and processing NMR Software

Available Probes

• Bruker BBFO probe: 5 mm Double Resonance Broadband direct detection probe – optimized for both ¹H observation and X-nucleus observation (¹⁹F, ³¹P-¹⁰⁹Ag) with ¹H-decoupling; with automated tuning and matching
• Bruker SEX ²H probe: 5 mm Double Resonance Selective direct detection probe – for direct observation of deuterium (²H) with ¹H decoupling and fluorine (¹⁹F) lock
• Bruker HR-MAS TXI probe: 4 mm High Resolution Magic Angle Spinning (HR-MAS) triple resonance indirect detection probe – for multi-dimensional experiments at ¹H, ¹³C, and ¹⁵N frequencies, on viscous samples or biological tissues

The 400 MHz Bruker Avance III Spectrometer is equipped with 3 radio-frequency observe channels, ZZ-axis pulsed field gradients (PFG), and high power amplifier (60 A). It is highly suited for routine 1D and 2D NMR experiments of small molecules and diffusion experiments.

Spectrometer Information

• 400 MHz Bruker Avance III HD Spectrometer (9.4 T magnet)
• 3 radio-frequency observe channels
• GREAT60 amplifier for high power diffusion experiments
• ZZ-axis pulsed field gradients (PFG)
• automatic sample changer
• sample temperature control (-20 ºC to 150 ºC)
• TopSpin3 acquisition and processing NMR Software

Available Probes

• Bruker BBFO probe: 5 mm Double Resonance broadband direct detection probe – optimized for both ¹H observation and X-nucleus observation (¹⁹F, ³¹P-¹⁰⁹Ag) with ¹H-decoupling; with automated tuning and matching
• Bruker TXI probe: 5 mm Triple Resonance indirect detection probe – optimized for ¹H-detected triple resonance (¹H, ¹³C, ¹⁵N) experiments; with automated tuning and matching
• Bruker DiffBB probe: 5 mm high power (gradient strength up to 17 T/m) diffusion BBI ¹H and ¹⁹F probe; with automated tuning and matching

Requisition Form

The 20 MHz Bruker mq20 Relaxometer is based on Time Domain (TD) NMR. TD-NMR is an inherently quantitative method, insensitive to matrix effects that occur in many complex materials, and widely used in Quality Control (QC). TD-NMR provides clear advantages over wet-chemical and other spectroscopic methods.

Spectrometer Information

• Bruker mq20 20 MHz Relaxometer
• Bruker Minispec software
• sample temperature control
• 10 mm diameter sample tubes

Electron Paramagnetic Resonance (EPR) spectroscopy, also called Electron Spin resonance (ESR) spectroscopy, is a powerful technique to study paramagnetic ions and molecules (chemical species with unpaired electrons), for example, metal complexes and organic radicals.

Areas of EPR application include coordination chemistry, metallo-organic compounds, electrochemistry, redox processes, reaction kinetics involving radical intermediates, oxidative stability and antioxidant capacity of foods, active site structure and reaction mechanisms of enzymes, quantitation of reactive oxygen and nitrogen species (ROS/RNS), and many others.

Spectrometer Information

• Bruker EMX EPR Spectrometer
• Microwave Frequency Range: CW X-Band (9.08 – 9.88 GHz)
• X-Band Solid State Ultra Low Noise Microwave Bridge
  - Max leveled source output: 200 mW
  - Max. attenuation: 60 dB
  - Tuning range: 60 MHz
  - Integrated Bridge Controller w/ real time display for optimizing parameters
• Cavities for room temperature experiments
• Nitrogen and Helium cryostats for VT and Low T experiments
• Simulation Software: Sinfonia