RELEAF

Abstract

Confronting global environmental challenges, the RELEAF project breaks barriers in the food sector by adopting cost-effective and eco-friendly processes to create innovative nutraceuticals and functional foods from crop residues with enhanced efficacy. Originating from the INOVC2020 initiative, RELEAF has developed a pioneering bio-based processing technology that transforms blueberry agrowaste into sustainable next-generation prebiotics with immunonutritional benefits. The project’s potential applications are broad, particularly in addressing autoimmune central nervous system disorders such as Multiple Sclerosis (MS), which imposes a severe socioeconomic burden and significantly impacts quality of life.

By combining ecopharmacognosy extraction techniques and biotechnology, RELEAF leverages eco-innovative methods optimized for senescent blueberry leaves, enabling the recovery of bioactive compounds - specifically non-extractable polyphenolic metabolites - typically lost in conventional processing. The team aims to generate robust evidence by progressively demonstrating the mechanistic effects and functionality of these compounds, from in vitro assays and in vivo models to advanced ex vivo studies using fecal samples from MS donors, ultimately paving the way for clinical evaluation through Yoggyleaf, an unrefrigerated yogurt-based shake combining shelf stability, convenience, and scientifically validated health benefits.

RELEAF drives science-based innovation in the agrifood sector by valorizing abundant and affordable primary residues left in the field after blueberry harvesting, rooted in circular economy principles. The project is anchored in Sever do Vouga, the national capital of blueberries in central Portugal, and thrives through collaboration between IPN - one of the world’s top 10 innovation incubators - and the local hub Vouga Park. Together, they combine advanced technologies and specialized regional expertise. Under the banner “Releaf: turning waste into w(h)ealth,” the initiative aims to promote healthier dietary habits, improve well-being, and foster economic development and territorial cohesion across the region.