Catalog

46 services available

Culture medium

Ready-to-use culture media or concentrated reagent kits (BG11, M7, S2T2, F/2 with different salinities, LC, Z8, MHY) Soil and Sphagnum extract for M7, S2T2, and LC media. The composition of the culture medium is sent with the medium.

Deposit of microalgae and cyanobacteria

Public deposit of microalgae and cyanobacteria

Determination of monosaccharides (HPLC-RI)

Extraction, hydrolysis, identification, and quantification of monosaccharides from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-RI).

Determination of pigments (HPLC-DAD)

Extraction, identification and quantification of pigments from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-DAD)

Determination of the fatty acids profile (GC-FID)

Extraction, identification and quantification of fatty acids from microalgae and cyanobacteria by gas chromatography (GC-FID).

Determination of total lipid and lipid fractions

Extraction and quantification of total lipid from microalgae and cyanobacteria by gravimetric methods; separation and quantification of neutral lipids, glycolipids and phospholipids by column chromatography

Determination of total sugars

Extraction and quantification of total sugars of microalgae and cyanobacteria by gravimetric analysis and spectrophotometry

Genomic DNA of microalgae and cyanobacteria from the collection

Genomic DNA from microalgae and cyanobacteria from collection, minimum of 2 µg of DNA. Qualitative and quantitative assessment by spectrophotometry.

Isolation and preservation

Isolation and preservation of microalgae and cyanobacteria (routine subculture and cryopreservation)

Taxonomic identification of microalgae and cyanobacteria

Taxonomic identification of microalgae and cyanobacteria using a polyphasic approach (microscopy, molecular biology, biochemistry).

Culture medium

Ready-to-use culture media or concentrated reagent kits (BG11, M7, S2T2, F/2 with different salinities, LC, Z8, MHY) Soil and Sphagnum extract for M7, S2T2, and LC media. The composition of the culture medium is sent with the medium.

Deposit of microalgae and cyanobacteria

Public deposit of microalgae and cyanobacteria

Determination of monosaccharides (HPLC-RI)

Extraction, hydrolysis, identification, and quantification of monosaccharides from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-RI).

Determination of pigments (HPLC-DAD)

Extraction, identification and quantification of pigments from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-DAD)

Determination of the fatty acids profile (GC-FID)

Extraction, identification and quantification of fatty acids from microalgae and cyanobacteria by gas chromatography (GC-FID).

Determination of total lipid and lipid fractions

Extraction and quantification of total lipid from microalgae and cyanobacteria by gravimetric methods; separation and quantification of neutral lipids, glycolipids and phospholipids by column chromatography

Determination of total sugars

Extraction and quantification of total sugars of microalgae and cyanobacteria by gravimetric analysis and spectrophotometry

Genomic DNA of microalgae and cyanobacteria from the collection

Genomic DNA from microalgae and cyanobacteria from collection, minimum of 2 µg of DNA. Qualitative and quantitative assessment by spectrophotometry.

Isolation and preservation

Isolation and preservation of microalgae and cyanobacteria (routine subculture and cryopreservation)

Taxonomic identification of microalgae and cyanobacteria

Taxonomic identification of microalgae and cyanobacteria using a polyphasic approach (microscopy, molecular biology, biochemistry).

Elemental Analysis

Mass percentage (% m/m) quantification of carbon (C), hydrogen (H), nitrogen (N), sulfur (S) and oxygen (O) present in a sample.

Identification and Quantification of Ions

Quantitative and qualitative determination of ions (anions and cations).

Identification and/or Quantification of Compounds - Chromatographic Methods

Separation of the various components of a mixture, with the aim of identifying and quantifying these components.

Identification and/or Quantification of Compounds - Spectroscopic Techniques

Spectroscopic analysis methods consist of measuring the amount of radiation emitted or absorbed by molecules.

Rheology and Viscosity Analysis

Study of the deformational behavior and the flow of matter subjected to stress, under certain thermodynamic conditions over a period of time. It includes properties such as: elasticity, viscosity and plasticity.

Stability and Particle Size Tests

Determination of particle sizes (from 3 nm to 5000 μm). Aggregation, agglomeration and flocculation studies.

Thermal Analysis

Study of the behavior of materials as a function of time or temperature, when heated, cooled or kept at a constant temperature.

Analysis of Fatty Acids (GC, MIDI)

Bacterial Fatty Acids identification and quantification by Gas Chromatography using the standard MIS Library Generation Software (Sherlock Microbial ID System).

Analysis of Polar Lipids (TLC)

Analysis of Polar Lipids from bacterial strains by Thin Layer Chromatography.

Analysis of Respiratory Quinones (HPLC)

Analysis of Respiratory Quinones from bacterial strains by High Performance Liquid Chromatography.

Bacterial Strains from Public Collection

Bacterial Strains from Public Collection: Active cultures | Freeze-dried strains

Beer Analysis

Bacteria detection on WLD selective medium | Bacteria identification | Hemocytometer yeast cell count | Beer specific gravity at 20 °C | pH | Total acidity | Color on SRM scale | Color on EBC scale | Beer bitterness | Total reducing sugars

Biochemical Analysis

Analysis of fatty acids (GC, MIDI) | Analysis of polar lipids (TLC) | Analysis of respiratory quinones (HPLC)

Bioinformatic Services

Whole genome analysis | Phylogenomic analysis

Deposit of Bacterial Strains

Public Deposit | Safe Deposit | Renew the Safe Deposit Service | Annual Maintenance Fee | Release of Strain

Identification and Characterization

Genomic DNA Extraction | Sequencing of 16S rRNA gene | Phylogenetic analysis | Sequence Analysis of Genes | Mol% G+C Content Analysis of Genomic DNA | RAPD Fingerprinting for two Strains | Whole Genome Sequencing of one Prokaryotic strain

Isolation and Preservation

Isolation from a mixed culture plate | Preservation in −80 °C

Microbial Diversity

Denaturing Gradient Gel Electrophoresis (DGGE) | Phospholipid Fatty Acid Analysis (PLFA) | Barcoding Sequencing

Other Services

Multiproposes certificates (not related with describing of novel species) | Consultancy | Training and related services

Phenotypic Characterization

Basic Phenotypic Characterization (based on API® strips) | BIOLOG Microplates (for extensive testing of oxidation of different carbon sources) | Morphological study (celular morphologyl, Gram, KOH, catalase, oxidase)

Access to Widefield and Confocal Microscopes

Widefield Microscope with LED-based system (UV 385/30 nm, B 469/38 nm, G 555/30 nm, Y 590/27 nm, and R 631/33 nm) Scanning Confocal Microscope with 4 laser units (Diode 405 nm; Argon/2: 458, 488, 514 nm; DPSS: 561 nm and HeNe: 633 nm)

Microscopy Analysis by Light or Electron Microscopy

Observation and analysis of live or fixed samples.

Sample Processing for Electron Microscopy

The iLAB performs and provides the required materials and equipments necessary for sample processing for electron microscopy including fixation, dehydration, resin embedding and ultramicrotomy.