ACOI - Coimbra Collection of Algae

Culture medium

Ready-to-use culture media or concentrated reagent kits (BG11, M7, S2T2, F/2 with different salinities, LC, Z8, MHY) Soil and Sphagnum extract for M7, S2T2, and LC media. The composition of the culture medium is sent with the medium.

Deposit of microalgae and cyanobacteria

Public deposit of microalgae and cyanobacteria

Determination of monosaccharides (HPLC-RI)

Extraction, hydrolysis, identification, and quantification of monosaccharides from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-RI).

Determination of pigments (HPLC-DAD)

Extraction, identification and quantification of pigments from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-DAD)

Determination of the fatty acids profile (GC-FID)

Extraction, identification and quantification of fatty acids from microalgae and cyanobacteria by gas chromatography (GC-FID).

Determination of total lipid and lipid fractions

Extraction and quantification of total lipid from microalgae and cyanobacteria by gravimetric methods; separation and quantification of neutral lipids, glycolipids and phospholipids by column chromatography

Determination of total sugars

Extraction and quantification of total sugars of microalgae and cyanobacteria by gravimetric analysis and spectrophotometry

Genomic DNA of microalgae and cyanobacteria from the collection

Genomic DNA from microalgae and cyanobacteria from collection, minimum of 2 µg of DNA. Qualitative and quantitative assessment by spectrophotometry.

Isolation and preservation

Isolation and preservation of microalgae and cyanobacteria (routine subculture and cryopreservation)

Microalgae and cyanobacteria strains from the collection

Microalgae and cyanobacteria live strains

Taxonomic identification of microalgae and cyanobacteria

Taxonomic identification of microalgae and cyanobacteria using a polyphasic approach (microscopy, molecular biology, biochemistry).