/ Structures

ACOI - Coimbra Collection of Algae

Coimbra Collection of Algae (ACOI) is a microbiological resource center with expertise in the areas of biodiversity, cryopreservation, molecular biology and microalgae biotechnology.

Websitehttps://www.uc.pt/uc-blue
E-mailucblue@uc.pt

20 available services

Ammonia analysis in liquid matrices

 Ammonia Determination by Segmented Continuous Flow with Molecular Absorption Spectrometry
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Benthic Macroinvertebrates

 Determination and identification by species of the existing benthic community in sediment samples
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Culture medium

 Ready-to-use culture media or concentrated reagent kits (BG11, M7, S2T2, F/2 with different salinities, LC, Z8, MHY) Soil and Sphagnum extract for M7, S2T2, and LC media. The composition of the culture medium is sent with the medium.
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Deposit of microalgae and cyanobacteria

 Public deposit of microalgae and cyanobacteria
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Determination of monosaccharides (HPLC-RI)

 Extraction, hydrolysis, identification, and quantification of monosaccharides from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-RI).
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Determination of pigments (HPLC-DAD)

 Extraction, identification and quantification of pigments from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-DAD)
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Determination of the fatty acids profile (GC-FID)

 Extraction, identification and quantification of fatty acids from microalgae and cyanobacteria by gas chromatography (GC-FID).
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Determination of total lipid and lipid fractions

 Extraction and quantification of total lipid from microalgae and cyanobacteria by gravimetric methods; separation and quantification of neutral lipids, glycolipids and phospholipids by column chromatography
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Determination of total sugars

 Extraction and quantification of total sugars of microalgae and cyanobacteria by gravimetric analysis and spectrophotometry
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Genomic DNA of microalgae and cyanobacteria from the collection

 Genomic DNA from microalgae and cyanobacteria from collection, minimum of 2 µg of DNA. Qualitative and quantitative assessment by spectrophotometry.
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Isolation and preservation

 Isolation and preservation of microalgae and cyanobacteria (routine subculture and cryopreservation)
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Microalgae and cyanobacteria strains from the collection

 Microalgae and cyanobacteria live strains
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Nitrate analysis in liquid matrices

 Determination of Nitrates by Segmented Continuous Flow with Molecular Absorption Spectrometry
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Nitrite analysis in liquid matrices

 Determination of Nitrites by Segmented Continuous Flow with Molecular Absorption Spectrometry
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Phosphate Analysis in liquid matrices

 Determination of Phosphate by Segmented Continuous Flow with Molecular Absorption Spectrometry
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Silica Analysis in Liquid Matrices

 Determination of Silica by Segmented Continuous Flow with Molecular Absorption Spectrometry
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Stable Isotope Analysis (SIA)

 Determination of Stable Isotopes by Mass Spectrometry (δ 13C (‰); δ 15N (‰); % C; % N)
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Taxonomic identification of microalgae and cyanobacteria

 Taxonomic identification of microalgae and cyanobacteria using a polyphasic approach (microscopy, molecular biology, biochemistry).
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Total Nitrogen Analysis in liquid matrices

 Determination of Total Nitrogen by Segmented Continuous Flow with Molecular Absorption Spectrometry
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Total Phosphorus Analysis in liquid matrices

 Determination of Total Phosphorus by Segmented Continuous Flow with Molecular Absorption Spectrometry
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