Microscopia Confocal

General overview

The laboratory provides technical support on various microscope techniques and live cell imaging.

The unit currently stands with a Confocal Microscope LSM 710 Zeiss, as well as other fluorescence microscopes (Leica DMIRE2 and Olympus CKX41) and a cell live imaging station from Leica (with temperature and CO2 controllers: temp control 37-2 digital and CO2-IR sensor CTI Controller 3700).

The system is available to all FMUC researchers, as well as for users external to FMUC. All users will need to contact the lab before planning any work or before any sample observation.

The services provided are:

- Discussion of the protocols

- Guidance and help in the preparation of samples

- Guidance in the manipulation of the confocal/fluorescence systems and acquisition of images

- Help in the interpretation of data

Booking and admission conditions

Until the online booking system is available, booking will be done by the technician responsible for the unit, using the contacts provided in this page.

First time users will receive training by the technician in charge for the equipment. The duration of the training will be adjusted according to the previous knowledge and progress of each individual user.

All users need to be registered before using the confocal system. In order to perform the registration, it is mandatory to fill the registration form available on this website.

All new users should contact the facility to discuss the needs and payment methods prior to the first appointment.

Confocal facility rates

The service fees consider the differentiation of four classes of users:

1 – FMUC and IBILI.CNC users

2 – Users external to FMUC and IBILI.CNC but that belong to the University of Coimbra

3 – Users from other public universities/institutes

4 – Users from private institutions; users from university/industry interfaces

Tabela de preços em euros e IVA não incluído.

Services will be charged in multiples of 30 minutes. Minimum reservation time is 1 hour.

Cancellation policy

Reservations may be cancelled without charge up to 24 hours prior to the scheduled time.

Cancelling a reservation within 24 hours prior to the start time must be made by emailing hmgirao@fmed.uc.pt or calling to 239 480 221 (Dr. Henrique Girão).

A 1-hour fee is charged for reservations cancelled within less than 24 hours of the scheduled appointment.

No-shows or appointments cancelled after the scheduled time are charged for the full duration of the appointment.

Hours of operation

9/10 am – 5/6 pm from Monday to Friday.

Arrangements can be made for overnight, weekend or other scheduled imaging. Please contact the manager for more details.

Facility location

IBILI – 2nd floor

Subunit III, Faculty of Medicine, University of Coimbra

Azinhaga de Santa Comba, Celas

3000-548

Coimbra

Contacts

Director of the facility:

Dr. Henrique Girão (hmgirao@fmed.uc.pt)

Tel. 239 480 221

Fax. 239 480 280